Table 1 . Characteristics of P. aeruginosa strains isolated from clinical cases and environmental water.

Strain N°

Source (a)

Type of ward (b)

Hospital

City

O Serotype (c)

Date of isolation (d)

(day / month / year)

1

Pneumonia

ICU 1

A

I

1

13 / 05 /96

2

Pneumonia

ICU 1

A

I

8

06 / 11 / 96

3

Pneumonia

ICU 2

A

I

4

02 / 08 / 96

4

Pneumonia

ICU 2

A

I

1

30 / 08 / 96

5

Pneumonia

ICU 2

A

I

6

17 / 07 / 96

6

Pneumonia

ICU 2

A

I

6

25 / 02 / 97

7

Pneumonia

ICU 2

A

I

10

07 / 10 / 96

8

Pneumonia

ICU 3

A

I

PO

11 / 12 / 96

9

Pneumonia

ICU 3

A

I

3

26 / 02 / 97

10

Pneumonia

ICU 3

A

I

6

09 / 01 / 97

11

Pneumonia

ICU 3

A

I

F

13 / 02 / 97

12

Pneumonia

ICU 3

A

I

PO

05 / 06 / 97

13

Pneumonia

ICU 3

A

I

6

17 / 09 / 97

14

Pneumonia

ICU

B

II

4

14 / 05 / 97

15

Pneumonia

ICU

B

II

11

16 / 06 / 97

16

Pneumonia

ICU

B

II

6

14 / 09 / 97

17

Pneumonia

ICU

B

II

6

07 / 08 / 97

18

Pneumonia

ICU

C

III

12

16 / 07 / 97

19

Pneumonia

ICU

C

III

NO

01 / 04 / 97

20

Pneumonia

ICU

C

III

12

06 / 03 / 97

21

Pneumonia

ICU

C

III

1

26 / 03 / 97

22

Pneumonia

ICU

C

III

NO

26 / 03 / 97

23

Pneumonia

ICU

D

IV

11

UN

24

Pneumonia

ICU

D

IV

11

UN

25

Pneumonia

ICU

D

IV

6

UN

26

Pneumonia

ICU

D

IV

10

UN

27

Pneumonia

ICU

E

V

2

31 / 01 / 94

28

Pneumonia

ICU

E

V

6

08 / 03 / 94

29

Pneumonia

ICU

E

V

16

19 / 04 / 94

30

Pneumonia

ICU

E

V

2

03 / 05 / 94

31

Pneumonia

ICU

E

V

2

09 / 05 / 94

32

Pneumonia

ICU

E

V

6

18 / 05 / 94

33

Pneumonia

ICU

E

V

10

28 / 06 / 94

34

Pneumonia

ICU

F

VI

6

NP / 12 / 96

35

Pneumonia

ICU

F

VI

6

NP / 12 / 96

36

Pneumonia

ICU

F

VI

6

NP / 12 / 96

37

Bacteremia

Onc

G

VII

16

01 / 02 / 96

38

Bacteremia

Onc

G

VII

1

03 / 09 / 95

39

Bacteremia

Onc

G

VII

1

21 / 10 / 95

40

Bacteremia

Onc

G

VII

3

07 / 05 / 96

41

Bacteremia

Onc

G

VII

3

07 / 08 / 95

42

Bacteremia

Onc

G

VII

6

17 / 07 / 96

43

Bacteremia

Onc

G

VII

16

26 / 04 / 96

44

Bacteremia

Onc

G

VII

6

21 / 01 / 97

45

Bacteremia

Onc

G

VII

11

28 / 11 / 96

46

Bacteremia

Onc

B

II

11

12 / 03 / 97

47

Bacteremia

Onc

B

II

PO

21 / 04 / 97

48

Bacteremia

Onc

B

II

PO

29 / 06 / 97

49

Bacteremia

Onc

H

IV

12

24 / 04 / 97

50

Bacteremia

Onc

I

I

11

04 / 07 / 97

51

Bacteremia

Onc

I

I

NO

02 / 12 / 97

52

Bacteremia

Onc

I

I

PO

08 / 10 / 97

53

Bacteremia

Onc

I

I

6

20 / 11 / 97

54

Bacteremia

Onc

I

I

11

30 / 06 / 97

55

Bacteremia

Onc

I

I

12

11 / 07 / 97

56

Bacteremia

Onc

I

I

10

12 / 08 / 97

57

Swimming pool

-

-

V

F

UN

58

Swimming pool

-

-

V

8

UN

59

Swimming pool

-

-

V

PO

UN

60

Swimming pool

-

-

V

8

UN

61

Swimming pool

-

-

V

8

UN

62

Swimming pool

-

-

V

6

UN

63

Swimming pool

-

-

V

1

UN

64

River 1

-

-

VIII

3

13 / 06 / 97

65

River 1

-

-

VIII

6

13 / 06 / 97

66

River 1

-

-

VIII

13

13 / 06 / 97

67

River 2

-

-

VIII

1

13 / 06 / 97

68

River 2

-

-

VIII

6

13 / 06 / 97

69

River 3

-

-

VIII

2

13 / 06 / 97

70

River 3

-

-

VIII

1

13 / 06 / 97

71

River 3

-

-

VIII

11

13 / 06 / 97

72

River 3

-

-

VIII

4

13 / 06 / 97

73

River 4

-

-

VIII

1

14 / 06 / 97

74

River 4

-

-

VIII

11

14 / 06 / 97

  1. Strains numbered 1 to 36 were isolated from cases of nosocomial pneumonia in ventilated patients, strains numbered 37 to 56, from cases of bacteremia in hospitalized cancer patients, and strains numbered 57 to 74, from rivers and swimming pools.
  2. ICU: Intensive care unit; Onc: oncology department.
  3. NO: Non agglutinable; PO: polyagglutinable; F: agglutinable with the O-serogroup F containing the antisera O7, O8, O11 and O12
  4. UN: unknown

Table 2. References Pseudomonas strains used.

Pseudomonas species

Source (a)

Strain

ATCC N°

O Serotype (b)

P. aeruginosa

CIP

CIP 5933

ATCC 33348

1

P. aeruginosa

CIP

CIP 5934

ATCC 33349

2

P. aeruginosa

CIP

CIP 5935

ATCC 33350

3

P. aeruginosa

CIP

CIP 5936

ATCC 33351

4

P. aeruginosa

CIP

CIP 5937

ATCC 33352

5

P. aeruginosa

CIP

CIP 5939

ATCC 33354

6

P. aeruginosa

CIP

CIP 5938

ATCC 33353

7

P. aeruginosa

CIP

CIP 5940

ATCC 33355

8

P. aeruginosa

CIP

CIP 5941

ATCC 33356

9

P. aeruginosa

CIP

CIP 5943

ATCC 33357

10

P. aeruginosa

CIP

CIP 5944

ATCC 33358

11

P. aeruginosa

CIP

CIP 5945

ATCC 33359

12

P. aeruginosa

CIP

CIP 6092

ATCC 33360

13

P. aeruginosa

CIP

CIP 7212

14

P. aeruginosa

CIP

CIP 7213

15

P. aeruginosa

CIP

CIP 100720 T

ATCC 10145 T

6

P. aeruginosa

ATCC

CIP 76110

ATCC 27853

6

P. stutzeri

CIP

CIP 103022 T

ATCC 17588 T

NO

P. putida

CIP

CIP 52.191 T

ATCC 12633 T

NO

(a) Collections from which the bacteria were obtained. ATCC: American Type Culture Collection (Rockville. Md); CIP: Collection Institut Pasteur (Paris, France).

(b) NO: Non agglutinable

TABLE 3 . Oligonucleotide primers used in RAPD(a) assays.

Name

Sequences

5’ 3’

GC %)

Reference

Pyo 208

ACG GCC GAC C

80

(23)

Pyo 241

GCC CGA GCG G

90

(23)

Pyo 272

AGC GGG CCA A

70

(23)

Pyo 275

CCG GGC AAG C

80

(23)

Pyo 277

AGG AAG GTG C

60

(23)

Pyo 287

CGA ACG GCG G

80

(23)

Pyo 15

GCA GGG TGT T

60

This work

Pyo B7

GGT GAC GAC G

70

This work

Pyo B10

CTG CTG GGA C

70

This work

(a) RAPD : Random Amplified Polymorphic DNA assay.