Table 1 . Characteristics of P. aeruginosa strains isolated from clinical cases and environmental water.
Strain N° |
Source (a) |
Type of ward (b) |
Hospital |
City |
O Serotype (c) |
Date of isolation (d) (day / month / year) |
1 |
Pneumonia |
ICU 1 |
A |
I |
1 |
13 / 05 /96 |
2 |
Pneumonia |
ICU 1 |
A |
I |
8 |
06 / 11 / 96 |
3 |
Pneumonia |
ICU 2 |
A |
I |
4 |
02 / 08 / 96 |
4 |
Pneumonia |
ICU 2 |
A |
I |
1 |
30 / 08 / 96 |
5 |
Pneumonia |
ICU 2 |
A |
I |
6 |
17 / 07 / 96 |
6 |
Pneumonia |
ICU 2 |
A |
I |
6 |
25 / 02 / 97 |
7 |
Pneumonia |
ICU 2 |
A |
I |
10 |
07 / 10 / 96 |
8 |
Pneumonia |
ICU 3 |
A |
I |
PO |
11 / 12 / 96 |
9 |
Pneumonia |
ICU 3 |
A |
I |
3 |
26 / 02 / 97 |
10 |
Pneumonia |
ICU 3 |
A |
I |
6 |
09 / 01 / 97 |
11 |
Pneumonia |
ICU 3 |
A |
I |
F |
13 / 02 / 97 |
12 |
Pneumonia |
ICU 3 |
A |
I |
PO |
05 / 06 / 97 |
13 |
Pneumonia |
ICU 3 |
A |
I |
6 |
17 / 09 / 97 |
14 |
Pneumonia |
ICU |
B |
II |
4 |
14 / 05 / 97 |
15 |
Pneumonia |
ICU |
B |
II |
11 |
16 / 06 / 97 |
16 |
Pneumonia |
ICU |
B |
II |
6 |
14 / 09 / 97 |
17 |
Pneumonia |
ICU |
B |
II |
6 |
07 / 08 / 97 |
18 |
Pneumonia |
ICU |
C |
III |
12 |
16 / 07 / 97 |
19 |
Pneumonia |
ICU |
C |
III |
NO |
01 / 04 / 97 |
20 |
Pneumonia |
ICU |
C |
III |
12 |
06 / 03 / 97 |
21 |
Pneumonia |
ICU |
C |
III |
1 |
26 / 03 / 97 |
22 |
Pneumonia |
ICU |
C |
III |
NO |
26 / 03 / 97 |
23 |
Pneumonia |
ICU |
D |
IV |
11 |
UN |
24 |
Pneumonia |
ICU |
D |
IV |
11 |
UN |
25 |
Pneumonia |
ICU |
D |
IV |
6 |
UN |
26 |
Pneumonia |
ICU |
D |
IV |
10 |
UN |
27 |
Pneumonia |
ICU |
E |
V |
2 |
31 / 01 / 94 |
28 |
Pneumonia |
ICU |
E |
V |
6 |
08 / 03 / 94 |
29 |
Pneumonia |
ICU |
E |
V |
16 |
19 / 04 / 94 |
30 |
Pneumonia |
ICU |
E |
V |
2 |
03 / 05 / 94 |
31 |
Pneumonia |
ICU |
E |
V |
2 |
09 / 05 / 94 |
32 |
Pneumonia |
ICU |
E |
V |
6 |
18 / 05 / 94 |
33 |
Pneumonia |
ICU |
E |
V |
10 |
28 / 06 / 94 |
34 |
Pneumonia |
ICU |
F |
VI |
6 |
NP / 12 / 96 |
35 |
Pneumonia |
ICU |
F |
VI |
6 |
NP / 12 / 96 |
36 |
Pneumonia |
ICU |
F |
VI |
6 |
NP / 12 / 96 |
37 |
Bacteremia |
Onc |
G |
VII |
16 |
01 / 02 / 96 |
38 |
Bacteremia |
Onc |
G |
VII |
1 |
03 / 09 / 95 |
39 |
Bacteremia |
Onc |
G |
VII |
1 |
21 / 10 / 95 |
40 |
Bacteremia |
Onc |
G |
VII |
3 |
07 / 05 / 96 |
41 |
Bacteremia |
Onc |
G |
VII |
3 |
07 / 08 / 95 |
42 |
Bacteremia |
Onc |
G |
VII |
6 |
17 / 07 / 96 |
43 |
Bacteremia |
Onc |
G |
VII |
16 |
26 / 04 / 96 |
44 |
Bacteremia |
Onc |
G |
VII |
6 |
21 / 01 / 97 |
45 |
Bacteremia |
Onc |
G |
VII |
11 |
28 / 11 / 96 |
46 |
Bacteremia |
Onc |
B |
II |
11 |
12 / 03 / 97 |
47 |
Bacteremia |
Onc |
B |
II |
PO |
21 / 04 / 97 |
48 |
Bacteremia |
Onc |
B |
II |
PO |
29 / 06 / 97 |
49 |
Bacteremia |
Onc |
H |
IV |
12 |
24 / 04 / 97 |
50 |
Bacteremia |
Onc |
I |
I |
11 |
04 / 07 / 97 |
51 |
Bacteremia |
Onc |
I |
I |
NO |
02 / 12 / 97 |
52 |
Bacteremia |
Onc |
I |
I |
PO |
08 / 10 / 97 |
53 |
Bacteremia |
Onc |
I |
I |
6 |
20 / 11 / 97 |
54 |
Bacteremia |
Onc |
I |
I |
11 |
30 / 06 / 97 |
55 |
Bacteremia |
Onc |
I |
I |
12 |
11 / 07 / 97 |
56 |
Bacteremia |
Onc |
I |
I |
10 |
12 / 08 / 97 |
57 |
Swimming pool |
- |
- |
V |
F |
UN |
58 |
Swimming pool |
- |
- |
V |
8 |
UN |
59 |
Swimming pool |
- |
- |
V |
PO |
UN |
60 |
Swimming pool |
- |
- |
V |
8 |
UN |
61 |
Swimming pool |
- |
- |
V |
8 |
UN |
62 |
Swimming pool |
- |
- |
V |
6 |
UN |
63 |
Swimming pool |
- |
- |
V |
1 |
UN |
64 |
River 1 |
- |
- |
VIII |
3 |
13 / 06 / 97 |
65 |
River 1 |
- |
- |
VIII |
6 |
13 / 06 / 97 |
66 |
River 1 |
- |
- |
VIII |
13 |
13 / 06 / 97 |
67 |
River 2 |
- |
- |
VIII |
1 |
13 / 06 / 97 |
68 |
River 2 |
- |
- |
VIII |
6 |
13 / 06 / 97 |
69 |
River 3 |
- |
- |
VIII |
2 |
13 / 06 / 97 |
70 |
River 3 |
- |
- |
VIII |
1 |
13 / 06 / 97 |
71 |
River 3 |
- |
- |
VIII |
11 |
13 / 06 / 97 |
72 |
River 3 |
- |
- |
VIII |
4 |
13 / 06 / 97 |
73 |
River 4 |
- |
- |
VIII |
1 |
14 / 06 / 97 |
74 |
River 4 |
- |
- |
VIII |
11 |
14 / 06 / 97 |
Table 2. References Pseudomonas strains used.
Pseudomonas species |
Source (a) |
Strain |
ATCC N° |
O Serotype (b) |
P. aeruginosa |
CIP |
CIP 5933 |
ATCC 33348 |
1 |
P. aeruginosa |
CIP |
CIP 5934 |
ATCC 33349 |
2 |
P. aeruginosa |
CIP |
CIP 5935 |
ATCC 33350 |
3 |
P. aeruginosa |
CIP |
CIP 5936 |
ATCC 33351 |
4 |
P. aeruginosa |
CIP |
CIP 5937 |
ATCC 33352 |
5 |
P. aeruginosa |
CIP |
CIP 5939 |
ATCC 33354 |
6 |
P. aeruginosa |
CIP |
CIP 5938 |
ATCC 33353 |
7 |
P. aeruginosa |
CIP |
CIP 5940 |
ATCC 33355 |
8 |
P. aeruginosa |
CIP |
CIP 5941 |
ATCC 33356 |
9 |
P. aeruginosa |
CIP |
CIP 5943 |
ATCC 33357 |
10 |
P. aeruginosa |
CIP |
CIP 5944 |
ATCC 33358 |
11 |
P. aeruginosa |
CIP |
CIP 5945 |
ATCC 33359 |
12 |
P. aeruginosa |
CIP |
CIP 6092 |
ATCC 33360 |
13 |
P. aeruginosa |
CIP |
CIP 7212 |
14 |
|
P. aeruginosa |
CIP |
CIP 7213 |
15 |
|
P. aeruginosa |
CIP |
CIP 100720 T |
ATCC 10145 T |
6 |
P. aeruginosa |
ATCC |
CIP 76110 |
ATCC 27853 |
6 |
P. stutzeri |
CIP |
CIP 103022 T |
ATCC 17588 T |
NO |
P. putida |
CIP |
CIP 52.191 T |
ATCC 12633 T |
NO |
(a)
Collections from which the bacteria were obtained. ATCC: American Type Culture Collection (Rockville. Md); CIP: Collection Institut Pasteur (Paris, France).(b)
NO: Non agglutinableTABLE 3 . Oligonucleotide primers used in RAPD(a) assays.
Name |
Sequences 5’ 3’ |
GC %) |
Reference |
Pyo 208 |
ACG GCC GAC C |
80 |
(23) |
Pyo 241 |
GCC CGA GCG G |
90 |
(23) |
Pyo 272 |
AGC GGG CCA A |
70 |
(23) |
Pyo 275 |
CCG GGC AAG C |
80 |
(23) |
Pyo 277 |
AGG AAG GTG C |
60 |
(23) |
Pyo 287 |
CGA ACG GCG G |
80 |
(23) |
Pyo 15 |
GCA GGG TGT T |
60 |
This work |
Pyo B7 |
GGT GAC GAC G |
70 |
This work |
Pyo B10 |
CTG CTG GGA C |
70 |
This work |
(a)
RAPD : Random Amplified Polymorphic DNA assay.